Simple Microplate Method for Determination of Urinary Iodine

Simple microplate method for determination of urinary iodine.

BACKGROUND Urinary iodine is a good biochemical marker for control of iodine deficiency disorders. Our aim was to develop and validate a simple, rapid, and quantitative method based on the Sandell-Kolthoff reaction, incorporating both the reaction and the digestion process into a microplate format. METHODS Using a specially designed sealing cassette to prevent loss of vapor and cross-contamin...

Rapid acid digestion and simple microplate method for milk iodine determination.

Iodine deficiency leads to deficiency of thyroid hormones, which causes mental retardation in infant. Laboratory confirmation is important in its diagnosis. The major problems associated with the existing methods for iodine determination in milk samples are: 1) nonsafe alkaline solution; 2) harsh thermal condition; and 3) extra time required to complete various steps. In this study, a simple an...

A simple method for determination of plasma and urinary biotin.

Measurement of biotin in plasma and urine has been stimulated by recent descriptions of inborn errors of biotin metabolism and by newly recognized causes of biotin deficiency. Biotin determination in physiologic fluids to document these conditions has been hindered by lack of a widely useable assay. This paper presents a method which employs tritium-labelled biotin, avidin, and nitrocellulose f...

A Simple Gravimetric Method for the Determination of Urinary Potassium

A simple method for the determination of serum cholesterol